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SEMEN  QUALm  AND  LIBIDO  OP  RAl-IS  OF  DIFFEBEOT  I-50TT0N  BREEDS 

THE  Smmfi  MONTHS 


by 


TED  H.  DOAJIB 


B»  S,,  Oklahoma  Agricultural  and  Mechanical  College, 
Stillwater i  Ofclahotoa,  1952 


A  THESIS 

submitted  in  partial  fulfilliaent  of  the 

requirements  for  the  degree 

MASTER  OS'  SCIENCE 

Department  of  ..\nlmal  Husbandry 

KANSAwS  STATE  COLLEGE 
OP  AGHICTJLTUR3  AIJD  APPLIED  SGIENCB 

1953 


Docu- 
ment 


ii 


TABLE  01?  COKTEOTS 


Q        IIITRODUCTION  1 

LITERATURE  REVIEW  2 

BXPr-RE'ElITAL  PROCEDURES  ,  ^ 

Animals  Used , , . ,  , ,   ? 

ISathods  of  Determining  Valuation  of  Libido...  ? 

Collection  of  Seosen.  ♦ . . .  .Il2 

Method  of  Determining  Motility..  ..17 

Method  of  Counting  Spero^    17 

EXPERIMENTAL  RESULTS  21 

SUMMARY  AIJD  CONCLUSIONS. . ,  28 

AC:aiO\\CLEDGEMEira   .30 

LITSRATURB  CITED  ...31 

APP3I\[DIX  32 


m 


PRODUCTION 


Sheep  production  has  been  an  important  livestock  production 
enterprise  in  the  United  Str.tes  for  many  years.    Ranchers  and 
farmers  have  made  many  changes  in  production  methods  during  this 
time  to  make  it  more  profitable. 

Lamh  production  is  influenced  to  a  large  extent  by  the 
reproductive  efficiency  of  the  males  and  females;  therefore,  it 
is  very  important  that  the  sheep  raiser  know  more  about  the  nor- 
mal reproductive  process  of  sheep  so  he  can  secure  maximum  lamb 
crops. 

Most  breeds  of  sheep  breed  only  during  late  summer  and  fall. 
Breeding  at  this  time  permits  the  birth  of  lambs  from  December 
to  March  and  even  on  into  the  sumrcer  months.    Many  farmers  and 
ranchers  in  Kansas  and  other  parts  of  the  United  States  prefer 
that  lambs  be  dropped  as  early  in  the  fall  as  possible  so  the 
lambs  will  be  larg:e  enough  to  sell  before  the  hot  season  starts 
in  late  spring  and  sunmer.    The  top  seasonal  market  prices  are 
generally  obtained  in  early  spring  and  late  winter.  Early 
lambing  is  essential  If  these  are  to  be  obtained.    Kany  pure- 
bred sheep  breeders  prefer  fall  lambing  with  part  of  the  flock 
because  it  facilitates  the  use  of  young  males  for  sale,  showing, 
and  breeding. 

Since  the  gestation  period  of  the  domesticated  sheep,  genus 
Ovis,  is  approximately  150  days,  the  ewes  must  be  bred  in  May, 
June,  or  July  in  order  to  produce  lambs  early  enough  to  be 


2 


benefited  by  more  favorable  climatic  conditions  for  growth  as  well 
as  a  high  market  price* 

It  has  not  been  clearly  demonstrated  vrtiethcr  it  Is  entirely 

« 

the  fault  of  the  ewes  if  they  fail  to  produce  early  lambs.  Rams 
may  also  bo  responsible  if  there  is  a  decline  in  their  fertility 
during  surrjner  months.    One  of  the  purposes  of  this  experiment 
was  to  study  reproductive  potency  of  the  racs  of  mutton  breeds 
during  the  season  of  the  year  when  ewes  must  be  bred  to  produce 
fall  lambs. 

The  other  purpose  of  this  experiment  i/as  to  compare  the 
reproductive  potency  of  different  breeds  of  rams.    This  infor- 
mation would  help  the  commercial  breeder  to  select  a  breed  that 
has  a  high  breeding  efficiency  during  the  summer  months,  and  thus 
enable  him  to  secure  a  higher  lambing  percentage  during  the 
fall  lambing  season. 

LITERATURE  REVIEW 

Ifeny  farmers  and  ranchers  x/ho  raise  sheep  In  Kansas  and 
the  Southwestern  States  assume  that  rams  of  mutton  breeds  are 
poor  breeders  during  the  summer  months  of  June  and  July  because 
cf  the  seasonal  high  temperature. 

According  to  Rice  and  Andrews  (6),  high  environmental 
temperatures  may  cause  temporary  sterility,  and  perhaps  a  perma- 
nent sterility,  if  the  temporature  of  the  testis  is  elevated 
above  the  body  temperature  ^./hlch  is  approximately  105*^  P. 


1 


The  testes  are  very  sensitive  to  temperature  changes;  and  if 
the  scrotum  cannot  maintain  these  organs  at  the  optimum  tempera- 
ture which  is  102°  F,,  there  Is  a  physiological  disturbance  of 
normal  spermatogenesis  \jt\lQh  results  in  the  i^roduction  of  poor 
quality  semen.    The  fertility  of  the  ram  is  also  narkedly  re- 
duced.   An  atmospheric  temperature  of  110°-ll5^  F.  may  raise 
the  scrotal  temperature  to  a  point  at  vrhich  it  is  detrimental 
to  normal  spermatogenesis.    The  scrotuia  is  an  efficient  thermal 
regulator  in  the  presence  of  environmental  temperatures  lower 
than  102*^  P. 

The  insulation  provided  by  the  covering  of  wool  on  the 
scrotum  and  the  natural  position  of  the  scrotum,  which  is  near 
coolness  of  the  ground  on  humid  days,  provide  considerable 
protection  against  high  temperatures.    Another  factor  which  aids 
In  preventing  complete  sterility  is  the  normal  cooling  at  nl^ts. 

I'lcKenzle  and  Berliner  (2)  studied  semen  qualities  of 
Hampshire  and  Shropshire  rams  in  Missouri  and  reported  that 
spermatogenesis  was  continuous  during  all  months  of  the  year, 
but  noted  that  there  were  definite  seasonal  trends  In  the  quality. 
The  Hampshires  appeared  to  have  a  maximum  semen  quality  from 
August  to  January  and  that  for  the  Sbropshires  ranged  from 
October  to  January.    A  pronounced  reduction  in  semen  quality 
prevailed  during  the  months  of  July  and  August. 

According  to  Moore  and  Quick  (k) y  the  temperature  in  the 
scrotum  is  much  lower  than  that  of  the  peritoneal  cavity  and 


found  that  tho  difference  betwoen  the  temperature  of  these 
two  positions  changed  as  the  temperature  of  the  external  envi- 
ronment varied. 

Rams  possessing  a  large  breedinj?  capacity  generally  dis- 
play a  very  active  libido.    There  are  several  factors  which 
affect  the  sex  drive  of  a  male.    The  greatest  handicap  of  mutton 
rams  is  an  excessive  amount  of  fat.    McKenzie  and  Phillips  (3) 
state  that  rams  in  high  condition  are  sluggish  and  unwilling  to 
hread  on  decisively  warm  days*    The  mutton  rams  have  a  tendency 
to  become  fat  more  readily  than  other  types  of  rams 5  therefore, 
the  ration  of  mutton  rams  should  be  observed  more  closely. 

Libido  and  sexual  activity  are  very  similar,  and  it  is  very 
important  to  know  if  rams  have  the  abilit;>^  to  find  an  estrous 
ewe.    A  ram  should  also  be  able  to  make  several  breeding  services 
a  day  over  an  extended  period  of  time.    McKenzie  and  Berliner  (2) 
concluded  that  an  average  of  five  successive  mounts  by  a  ram 
during  a  ten-minute  period  was  indicative  of  excellent  libido. 

Sperm  motility  is  a  very  good  indicator  of  fertility.  If 
a  ram  does  not  have  active  sperm,  his  qualities  as  a  breeder  are 
poor.    Schott  and  Phillips  (7)  found  that  the  rate  of  movement 
of  the  noriaal  sperm  is  very  rapid.    '#ien  placed  in  a  proper 
media,  raammali?.  spermetazoa  v/ere  noted  to  move  from  1-^  mm  per 
minute,  v/ith  ac  average  of  3  mm  per  minute.    Phillips  and  Andrews 
(5)  found  ram  sperm  in  the  ovarian  bursa  (funnel)  of  the  ewe 
30  minutes  after  copulation. 


EXPERIKEKDAL  PROCEDORES 


Animals  Used 

The  sinimals  used  for  this  experiment;  v;ere  nine  purebred 
stud  rams  of  Kansas  State  Collese  (Plates  1,  2|  and  3)»  The 
groups  included  three  Hampshires,  three  Shropshires,  two  South- 
do-wns,  and  one  Suffolk,    They  were  fed  the  basic  ration  consist- 
ing of  one-half  oats,  one  fourth  bran,  and  one-fourth  corn  at 
the  rate  of  one  pound  per  ram  per  day.    Tlio  roughage  was  sudan 
pasture.    The  rams  were  housed  in  the  college  sheep  barn  during 
the  summer  of  1952  which  was  unusually  hot  and  dry.^ 

At  the  beginning  of  the  experisiental  period,  tiro  barren 
ewes  were  injected  with  a  natural  estrogenic  hormone,  Estrone, 
and  the  rams  were  permitted  to  mount  them  in  a  normal  manner* 
A  specially  constructed  chute  (Plate       Fig,  1)  retained  the 
females  relatively  motionless  during  breeding  or  collection  of 
semen.    The  rams  became  less  timid  after  a  series  of  preliminary 
mounts  were  accomplished. 

Methods  of  Deteraining  Valuation  of  Libido 

The  breeding  activity  of  all  rama  vas  observed  and  recorded 
so  that  comparisons  could  be  made,    A  series  of  ratings  v/ere 
used  to  classifjr-  all  rams.    Libido  way  rated  as  "excellent"  if 
the  ram  mounted  and  served  a  owe  prompt lyj  libido  was  rated  as 

1,    Average  daily  temperature  92°,  average  monthly  rainfall  1/2" 
to  compare  with  the  ten-year  average  temperature  800, 
rainfall  2".  ' 


EXPLMATIOH  OP  PLATE  I 


Figure  1  Hampshire  Ram  nuDioer  1  3  years  old 
Figure  2  Hampshire  Ran  number  2  2  years  old 
Figure  3     Hampshire  Ram  number  3       1  year  old 


PLATE  I 


EXPLAHATIOK  OP  PLATE  II 


Figure  1     Suffollr.  Ram 
Figure  2     Soutbdo\jn  Ram 
Figure  3     SouthdoLU  Ram 


2  3''ears  old 
number  1  2  years  old 
number  2      h  years  old 


9 


PLATE  II 


EXPLMATIOlj  OP  PLATE  III 


Figure  1     Shropshire  Rp.m  number  1       1  ;rear  old 
Figure  2     Shropshire  Rem  number  2       2  years  old 
Figure  3     Shropshire  Ran  number  3       3  years  old 


11 


PLATE  III 


"good"  if  he  mounted  and  served  within  three  minutes.  Libido 
was  rated  as  "fair"  if  the  ram  mounted  and  served  betv;een  three 
and  five  minutes.    If  the  ram  was  uninterested,  he  was  rated  as 
"poor".    Breeding  activity  and  libido  of  the  rams  was  also  ob- 
served upon  turning  them  into  a  flock  of  ewes  to  search  and  find 
those  in  heat.    The  following  ratings  were  given;    if  the  ram 
was  eager  in  his  efforts,  he  was  rated  as  "good";  if  he  was  slug 
gish  but  attempted  to  search,  he  was  given  a  "fair"  rating;  and 
if  he  was  entirely  uninterested,  he  was  classified  as  "poor"* 

Collection  of  Semen 

After  the  rams  were  familiar  with  breeding  the  ewes  in  the 
chute,  an  artificial  vagina  (Plate  5)  was  used  to  collect  the 
semen.    As  the  rams  mounted  the  ewes,  the  penis  v/as  guided  into 
the  artificial  vagina. 

The  artificial  vagina  was  made  from  a  number  2  automobile 
water  hose  (approximately  six  inches  long)  vrtiich  was  used  as  the 
outer  jacket  and  a  section  of  rubber  tubing  of  soft  flexible  ma- 
terial as  the  inner  lining.    A  valve  was  inserted  in  the  outer 
casing  to  enable  the  collector  to  regulate  air  pressure  between 
the  layers  (Plate  5,  Fig.  1,).    Water  of  approximately  110°-1200 
was  placed  between  the  two  tubings  and  enough  air  was  forced 
through  the  valve  to  produce  satisfactory  pressure  for  the  rams. 
A  lubricant  of  an  oil-base  gelatin  (KY  Jelly)  was  used  to  ensure 
free  motion  of  the  penis.    A  test  tube  was  attached  to  the  end 


EXPLANATION  OF  PLATE  IV 


Figure  1     Chute  constrncted  for  confinement  of  ewe. 
Figure  2     Ewe  confined  to  chute. 

Figure  3     Ran  approaching  ewe  in  chute  for  collection. 


EXPLMATION  OF  PLATS  V 


Figure  1     Unconatructed  artificial  vagina. 

Figtrre  2     Equipment  used  to  bring  about  estrous 
and  for  collection  of  semen. 


PLATE  V 


Fig.  2 


17 


of  the  inner  lining  by  a  funnel-shaped  rubber  tube  to  collect 
the  ejaculate  (Plate  5,  Fig.  2). 

Method  of  Determining  Motility 

Sperm  motility  was  observed  by  placing  a  drop  of  aeraen  at 
one  end  of  a  clean  miscroscope  slide.    The  drop  of  semen  was 
drawn  across  the  first  slide  as  to  make  an  even  film. 

This  smear  was  made  immediately  after  the  semen  collection 
to  prevent  the  sperm  from  dying  or  becoming  sluggish  due  to  light, 
temperature,  and  other  mechanical  disturbances.    The  slide  was  ex- 
amined with  a  miscroscope  under  high  pov;er  for  the   rhipping  and 
movement  of  the  sperm's  tails.    Motility  was  classified  by  num- 
bers ranging  from  1  to  5,  with  5  indicating  the  greatest  motility. 
If  wave  movements  were  seen  due  to  rapid  whipping  of  tails,  the 
sample  of  semen  was  classified  very  high,  number  5.    If  the  tails 
were  moving  a  reasonably  active  speed,  motility  was  rated  good  or 
k.    If  the  sperm  were  moving  at  a  progressive  movement  limited  to 
individual  sperm,  it  was  rated  fair  by  number  3.    If  they  were 
very  sluggish  and  moving  slowly,  they  were  classified  as  poor  or 
2 J  and  if  the  sperm  were  dead,  they  were  rated  as  1  which  meant  no 
motility* 

Method  of  Counting  Sperm 

A  Hemecytometer  (Plate  VI)  was  used  to  make  the  sperm  counts 
as  described  by  Bergman  and  Hewitt  (1)  for  counting  red  blood 
cells.    The  red  blood  cell  diluting  pipette  and  a  diluting  fluid 


EXPLANATION  OP  PLA'^  VX 


Equipcyant  uaod  for  oouriUing  of  sperm  and  for  determining 

fpotllity  of  the  gperm» 


of  2^%  sodium  ci irate  was  used  to  make  the  semen  dilution.  The 
technique  used  was  to  draw  semen  within  the  pipette  up  to  the  ,5 
mark  and  then  draw  the  diluting  fluid  into  the  pipette  up  to  the 
11  mark.    After  this  step,  the  index  finger  and  the  thumb  were 
placed  over  each  end  of  the  pipette  and  shaken  well  until  the  liq- 
uids were  thoroughly  mixed*    A  standard  counting  chamber  was  used. 
After  placing  a  cover  slip  of  the  region  of  the  slide  with  the 
marked  area,  a  drop  of  diluted  semen  was  placed  at  the  edge  of  the 
cover  slip  so  it  could  flow  under  the  cover  slip.    The  counting 
chamber  was  placed  under  high  power  objective  of  the  microscope. 
Five  blocks  were  counted  on  the  field  of  l6  squares.    These  were 
the  corners  and  the  middle  square  of  the  field. 

The  sum  of  these  squares  equalled  the  number  of  sperm  in  80 
small  squares.    The  small  squares  on  the  counting  chamber  covered 
an  area  of  one  square  nillimeter.    Since  each  small  square  equalled 
1/20  mm.  square,  it  took  ^0  squares  to  make  a  square  millimeter. 
Eighty  squares  equalled  1/5  square  ram.    The  depth  of  the  counting 
chamber  was  1/10  mm.  and  the  diluti  on  was  1/200.    Hence,  the  num- 
ber of  the  sperm  in  8o  small  squares  times  5  times  10  times  200 
equalled  the  number  of  sperm  per  cubic  millimeter  of  semen.  There- 
fore, 10,000  times  80  squares  equalled  the  number  of  sperm  per 
cubic  millimeter.    To  obtain  the  total  number  of  sperm  in  one  ejac- 
ulation, the  number  of  sperm  per  cubic  millimeter  was  multiplied 
times  100  and  the  product  was  multiplied  times  the  volume  of  semen 
the  individual  ejaculated. 


21 


EXPERIMENTAL  RESULTS 

The  rams  were  ranked  by  breed  and  within  "breeds  for  semen 
quality  and  libido* 

The  breeding  activity  of  the  Hampshires  and  Suffolk  in  the 
ewe  flock  was  high  regardless  of  the  extreme  temperature  which 
was  witnessed  during  the  collection  period.    The  Southdowns  and 
Shropshires  ranked  "good"  for  breeding  activity  in  the  ewe  flock. 

The  Suffolk  and  Hampshires  ranked  higher  than  the  Shrop- 
shires and  Southdotms  in  regard  to  motility. 

The  Suffolk  and  Hampshires  \/ere  never  below  the  "excellent" 
rating  (Table  1)  for  their  breeding  activity  upon  serving  a  ewe 
with  the  exception  of  four  times  when  the  Suffolk  vras  rather  tim- 
id and  one  yearling  Hampshire  was  still  inexperienced.    The  other 
exception  was  unexplainable • 

The  flock  breeding  activity  of  the  Southdown  and  Shropshire 
rams  were  very  similar.    One  ram  of  each  breed  was  very  active 
and  one  was  rated  "fair".    This  resulted  in  the  t\iro  breeds  receiv- 
ing a  "good"  rating.    The  ratings  of  the  four  breeds  of  rams  could 
be  in  two  groups i    "excellent"  for  the  Hampshires  and  Suffolk,  and 
"good"  for  Shropshires  and  Southdowns.    It  should  be  noted  that 
the  No.  3  Shropshire  was  ratea  "fair"  in  breeding  activity  al- 
though he  was  sterile. 

The  semen  characteristics  considered  for  its  evaluation  were 
volume,  number  of  sperm  per  cubic  millimeter,  total  number  of 


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23 


sperm  per  ejaculation  and  the  motility  of  aperm  (Table  ?.)• 

^lotility,  which  was  ranked  by  nucibers,  was  highest  in  the 
Hampshires  and  Suffolk.    The  Suffolk  received  the  highest  individ- 
ual motility  rating.    Motility  scores  for  the  three  Hampshire  rams 
showed  considerable  variability  but  on  the  average  were  higher 
than  the  mean  scores  of  the  Southdovms  and  Sliropshires.    The  semen 
from  these  two  later  breeds  received  an  average  motility  rating 
of  "fair". 

It  vras  found  that  the  sperm  count  of  the  Suffolk  was  800,000 
more  sperm  i^r  cubic  millimeter  than  the  next  highest  rated  indi- 
vidual \ihich  was  a  Southdown.    The  Suffolk's  average  number  of 
sperm  was  ^-,180, 000  to  the  No.  1  Southdown's  3>312jOOO  sperm/mm^. 
The  No.  1  axid  Ko.  2  Hampshires  were  also  rated  high  in  total  num- 
ber of  spf?rm  with  an  average  of  3>270,000  for  seven  collections 
and  3,260,000  for  eight  collections  respectively.    The  No.  3  Hamp- 
shire, Ho.  2  Southdovai  and  Wo.  2  Shropshire  had  approximately  the 
same  number  of  sperm  per  cubic  millimeter  of  semen.    The  range 
was  from  2,890,000  to  3>503,000.    The  No*  1  Shropshire,  while  being 
rated  "excellent"  in  his  activity  and  libido,  had  a  comparative  low 
sperm  count*    His  count  averaged  1,870,000  sperm  per  mm^  of  semen* 
The  No*  3  Shropshire  produced  only  dead  sperm j  however,  a  sperm 
count  was  taken  and  indicated  from  1,350,000  to  3,625,000  sperm  per 
mm-**    This  indicated  the  continuous  production  of  spermatozoa  even 
though  the  sperm  were  dead  at  the  time  of  e;5aculation. 

In  the  report  by  ItoKenzle  and  Berliner  (2),  the  volume  of 
semen  per  ejaculate  ranged  from  .6  to  1  cc.  per  ram.    There  were 


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a  few  exceptions  to  this  volume  range  in  the  semen  collections 
in  this  study.    In  four  collections,  semen  volumes  ranged, from 
2-2.50  cc.  per  ejaculation,  but  the  average  for  the  nine  rams  on 
kk  collections  was  1  cc.    The  Suffolk  surpassed  all  other  individ- 
ual with  1.625  cc.  per  collection.    This  vms  at  the  time  when  he 
was  not  being  used  for  any  breeding  except  for  this  experiment. 
The  exceptionally  high  volume  of  semen  per  ejaculation  for  this 
ram  may  be  explained  to  a  certain  extent  because  of  the  long  in- 
tervals between  each  collection.    The  reason  for  the  long  collec- 
tion intervals  was  because  he  often  became  timid  or  frightened, 
thus  delaying  the  collection  periods. 

The  Hampshires,  Shropshires,  and  So'ithdo^ms  ejaculated  ap- 
proximately 1  cc.  per  collection.    The  average  column  of  the  ejac- 
ulates vrere  1.073 »  •993 »  and  .965  respectively.    An  analysis  of 
varir.iiCG  was  calculated  for  semen  volume  as  described  in  Snedo- 
cor(8).    There  was  no  significance  found  between  breeds  and  within 
breeds. 

The  analysis  of  variance  of  semen  concentration  (Table  3)  re- 
vealed no  significance  bet\^reen  breeds,  but  there  was  a  highly  sig- 
nificant difference  between  rams.    This  is  shovm  in  the  analysis 
chart  v;ith  the  f  test  stated  at  ^.^6  at  the  5Jfa  level.    The  f  test 
was  8.3^  for  the  number  of  sperm  per  cubic  millimeter.    There  was 
no  significant  difference  between  rams  within  the  same  breed  fop 
motility,  but  for  the  test  betv/een  breeds  there  was  a  very  highly 
significant  difference,  with  the  f  test  of  5.12.    This  shows  that 
there  are  some  breeds  with  a  higher  semen  motility  than  others. 


Tatle  3»    Analysis  of  variance. 


D/P 


S3 


MS 


Voluaw 


Breed 

Rams,  Same  Br. 

Dates-RclSr. 

Total 


3 

1.3^v 

0M67 

0.96 
9.18 

0.1920 

0.2086 

52 

11.^8 

2.33  Ins. 
'..Ih  Ins. 


Breed 

26.28 

8.7600 

Rams,  Same  Br. 

i 

15.92 

3a8if0 

Dates-Ro-^r. 

16.79 

0,3816 

Total 

52 

58.99 

2.75  Ins. 
8.3^*** 


Breed 

Ra&s,  SajoB  Br. 

Dates-R<S:Br. 

Total 


3 
52 


61,71 
21.06 

17.68 
100.^-5 


20.5700 
^.2120 

^h,0l82 


5.12** 
Ins. 


27 


There  were  no  analyses  coEputed  for  libido  because  of  the 
very  similar  actions  of  rams  studied.    The  numbers  were  also  too 
small  to  Justify  analyses. 

The  ranking  of  the  rams  as  for  sperm  concentration  and  aver- 
age total  number  of  sperm  per  ejaculation  was  as  follows:    (1)  Suf- 
folk (6,782  billion);  (2)  Hampshires  (3.^391  billion);  (3)  South- 
dov/na  (2,8363  billion);  and  ik)  Shropshires  (1.7783  billion). 

In  suniiarizing  the  data  for  all  breeds,  the  Suffolk  was  most 
potent  as  a  breeder  in  the  sumEier  months.    The  Haapshirea  were 
second  and  louthdowns  third.    This  placed  the  Shropshires  last  ir- 
respective of  the  one  sterile  ram. 


28 


Sroa-IARS  AND  CONCLUSIONS 

The  reproductive  capacities  of  rams  of  different  mutton 
breeds  (Suffolk,  Hanpshire,  Shropshire,  and  Southdovms)  v/ore  stud- 
ied during  the  summer  months.    Most  of  the  experimental  data  was 
ottained  in  June  or  July;  however,  semen  collections  were  made  un- 
til Septemher  1^,  1952#    Three  Hampshires,  three  Shropshires,  two 
Southdovms  and  one  Suffolk  were  used  in  the  experiment* 

There  were  distinct  differences  among  the  mating  desires  of 
rams  of  different  breeds  as  well  as  bctiween  rams  within  the  sane 
breed*    The  Suffolk* s  and  Hampshires'  libido  were  i')ove  that  of 
the  Shropshires  and  Southdovjna  on  the  average.    The  sterility  of 
one  Shropshire  did  not  appear  to  influence  his  sex  desires. 

There  were  no  significant;  differences  in  the  production  of 
spern  due  to  the  effects  of  temperature  during  the  extremely  hot 
period.    The  sperm  number  did  not  vary  to  a  great  extent  as  to  in- 
dividual spermatogenesis. 

Itotility  scores  were  similar  for  all  ejaculates  of  individual 
rams.    There  were  three  rams  which  varied  more  than  tvro  points  on 
their  sperm  activity  scores.    The  Suffolk  v/as  most  constant  with 
an  "excellent"  rating  for  each  collection.    The  Hampshires  were 
second,  Southdo\«s  third,  and  the  Shropshires  were  last.  There 
was  a  wide  variation  in  No.  1  and  2  Hampshires  and  No.  1  South- 
downs,    Except  for  the  No.  3  JIampshire,  the  last  collection  was 
observed  to  have  the  highest  motility  rating  during  the  entire 
collection  period. 


29 


The  rams  could  be  divided  into  two  distinct  groups  for  breed- 
ing activity  and  semen  quality.    The  Hampshires  and  Suffolk  had 
the  highest  ratings.    The  Shropshires  and  Southdo;ms  were  rated 
somewhat  lower  than  the  tx^o  other  breeds.    U'hen  all  scores  were 
considered  on  an  equal  basis,  the  breeds  ranlced  as  follows i  Suf- 
folk, Hampshires,  Southdowns,  and  Shropshires. 

Late  lambing  from  summer  l;r3eding  did  not  appear  to  cause  an 
inability  of  the  rams  to  breed  the  ewes. 

There  are  also  indications  that  Suffolk  and  Hampshire  rams 
may  be  more  active  and  reliable  breeders  during  the  summer  months. 


ACKUOV/LBDGEllEKT 


The  author  wishes  to  express  his  Indebted  thariks  to 
Dr#  T.  Donald  Eell,  Head  of  the  Sheep  Departnent,  Animal  Hus- 
bandry, for  the  aid  and  direction  of  this  erpericient . 

For  the  careful  guidance  of  the  construction  and  criticism 
of  the  manuscript,  the  writer  extends  his  gratitude  to  Dr.  Bell 
and  also  Le-v/is  A,  Holland  ana  Vfelter  H,  Smith,  Assistant  Pro- 
fessors in  Animal  Husbandry* 

Acknov/ledgement  is  given  to  Tliomas  Dean,  Shepherd,  K*  S,  C 
for  his  aid  in  preparation  and  collection  of  the  data. 


3X 


LITF.nATTjRE  CITED 


(1)  Bergman,  H.  D.,  and  L.  A.  Hewitt. 

Laboratory  iianual  for  Experiment al  Physiology. 
Minneapolis!    B'lrgesa,  19*;-0.    l8  p. 

(2)  McKenzle,  F.  F.,  and  V,  R»  Berliner ^ 

The  Reproductive  Gapacjuj  of  Rams,    Mo.  Agr. 
Expt.  St a.    Hgs.  Bui.  265.  1937. 

(3)   ,  and  R.  v/.  Phillips. 

Measuring  Fertility  in  Hams.  Am.  Vet.  Med.  Asaoo. 
81+1109-202.  193^« 

(k)     I'foore,  C,  R.,  and  W.  J.  Quick. 

The  Scrotum  as  a  Temperature  Regulator  for  the 
Testis.    Am.  Jour.  Physiol.    68j70-79.  192^. 

(?)     Phillips,  R.  W,,  and  F.  N.  Andrews. 

The  Speed  of  Travel  of  Ram  Spermatozoa. 
Anat.  Rec.    68:127-132.  1937. 

(6)  Rice,  V.  A,,  and  F.  K.  Andrews. 

Breeding  and  Improvement  of  Farm  Animals. 
hth  ed.    Kew  York:    McGraw-Hill,  1950. 

(7)  Schott,  R.  G.,  and  R.  W.  Phillips. 

The  Rate  of  Sporm  Travel  and  Tiuie  of  Ovulation 
in  Sheep.    Anat.  Rec.    79 » 531-5^.  iM, 

(8)  Snedecor,  G.  W, 

Statistical  Methods,  ^th  ed.  Iowa  State  College 
Press.  19M-6. 


APPERDIX 


33 


APPEi®IX 


I)at9 


Volume 


no/mm3 


Total 


June  12 

June  2^ 

July  12 

July  22 
Aug  •  ^- 

Aug.  2h 

Sept*  l^f 

Total 
5 


Ilampshire  I 

1,00  hM 

1.50  3.85 

1.50  3.26 

1.00  3.70 

2.00  2.18 

1.00  2.58 

.75  2.91 

8.75  22.93 

1.25  3.27 


.775 
uC90 
3.700 
2.580 
2.580 
2.182 

27.937 
3.991 


June  12 

June  25 

July  2 

July  12 

July  22 

Aug.  k 

Aug.  2k 

Sept.  1^ 

Total 

mm 

X 


Hampshire  II 

1.50  3.51 

1.00  3.00 

1.00  3.00 

1.00  3.50 

1.00  3.56 

1.50  3.50 

1.00  2.91 

1.00  3.10 


9.00 
1.125 


26.08 
3.26 


5.265 
3.000 
3.000 
3.500 
3.560 
5.250 
2.910 
3.100 

29.585 
3.698 


ko 

5 


June  12 
June  25 
July  22 
Aug.  k 
Aug .  2^- 
Sept.  1^ 

Total 
I 


1.50 

.75 

1.00 
.66 

.75 
.50 

5.16 
.86 


Hampshire  III 

2.80 

2.77 
3.10 
2.67 
2.17 


17.356 
2.892 


5.760 

2.100 
2.776 
2.0if6 
2.002 
1.085 

15.769 
2.628 


h 

3 
2 

3 

21 

3.5 


Date 


Volume  No/Dan3 
(99 1),  J'^XlXm 


Total 


SaTfolk 


1  p 

h- 

July- 

1.2 

1.50 

6.750 

5 

July 

22 

2,00 

^.10 

8.200 

1 

Aug  • 

k 

Total 

6.5 

16.72 

27.130 

20 

5 

1.62? 

^.18 

6.782 

5 

Southdovjn  I 

June 

Ik 

1.50 

2.78 

^.170 

2 

June 

25 

.75 

3.00 

2.250 

3 

July 

2 

1.00 

4.10 

l+.lOO 

3 

July 

12 

1.00 

3.66 

3.660 

3 

Aug, 

.75 

3.50 

2.625 

2 

Aug. 

21+ 

1.00 

3.05 

3.050 

5 

Total 

7.00 

23.19 

22.955 

21 

1.00 

3.312 

3.179 

3 

Southdown  II 

June    25  1.00  1.50  1.500  2 

July     2  .66  ^.00  2.66M-  2 

July   12  2.50  2,25  5.625  3 

July   22  .50  1.76            .880  2 

Aug.      2  .63  2.10  1.323  2 

Aug.    2h  1.00  3.01  3.010  3 

Sept.  1^-  .50  2.90  1,450  3 

Total  6.79  17.52  17.^5  17 

2  .97  2,6^7  2,493 


Volume        Ilo/mm^  Total 
(fffii.)      '''llU9£i      MLUSZI  ?^1;lX:itiy 


Shropshlra  I 


Jane 
June 
July 

July 

12 

25 
2 
22 

1.00 
.50 
.50 

1.00 

2.10 

1.50 
1.68 
2.20 

2.100 

lo^ 

2.900 

Total 
X 

3.00 

.75 

7M 
1.87 

^.V59 

1.11 

3 
3 
3 
3 

12 
3 


July  2 

July  12 

July  22 

Aug.  h 

Total 


.2! 


.63 

2.50 
1.00 


1.0637 


Shropshire  II 

2.  ^1 

3.  ^ 
2.71 
3. BO 

11.32 
2.83 


.603 
2.125 
^.075 
2.850 

9.653 
2.^13 


3 
2 

3 
3 

11 
2.75 


June  12 
Juno  25 
July  22 
Aug. 

Aug.  2h 
Gept.  1^ 

Total 


1.00 
1.50 
1.00 

1.50 

1.00 
1.00 

7,00 
1.166 


Shropshire  III 

1.35 
1.75 


1.51 


1.350 
2.625 


I.Mk) 

5.^1-15 
1.805 


X 
1 
1 

1 
1 

1 

6 
I 


SEMEN  QUALITY  MD  LIBIDO  OP  RAJfS  OP  DIPPEREMT  l-IDTTON  BBEEDS 

IH  TliE  SUJxiJJi^;  MOOTS 

by 

TED  H.  DOME 

B.  S,,  Olclahoma  Agricultural  and  Mcch&nical  College 
Stillwater,  Oklahoma,  1952 


M  ABSTRACT  OP  A  MASTER'S  THESIS 

subnitted  in  partial  fulfillment  of  the 

requirements  for  the  degree 

MASTER  OF  SCEKCE 

Department  of  Animal  Husbandry 

KAI"ISAS  STATE  COLIEGE 
OF  AGRICULTTTHE  AiiD  APPLIED  SCISNCS 

1953 


Sheep  producers  in  the  United  States  are  confronted  with  a 
late  lainhing  problem.    This  means  the  lamhs  are  being  dropped  at 
a  time  of  year  ;^en  the  breeders  do  not  benefit  from  the  economi- 
cal advantages  which  occur.    The  factors  are  the  highest  seasonal 
trend  of  market  prices,  being  free  from  insects,  and  usage  of  ram 
lambs  for  breeding,  shoi^riLng,  and  selling  which  is  noticed  by  those 
who  produce  early  lambs.    To  acquire  these  benefits,  the  ewes  must 
be  bred  in  middle  summer  and  early  fall. 

One  of  the  purposes  of  this  experiment  was  to  investigate  the 
theory  that  rams  are  responsible  for  late  lambing  seasons  with  the 
temperature  of  the  summer  months  being  the  decisive  factor.  The 
other  purpose  was  to  compare  the  reproductive  potency  of  rams  of 
different  breeds  to  enable  the  commercial  breeder  to  select  a  breed 
uhloh  will  help  him  secure  a  higher  lambing  percentage  during  the 
early  lambing  season. 

Nine  rams,  including  three  tlampshires,  three  Shropshires,  two 
Southdowns,  and  one  Suffolk,  were  used  in  this  experiment. 

Semen  samples  were  collected  as  often  as  possible  between 
June  2,  1952,  and  September  1^,  1952,    The  saraples  were  analyzed 
for  motility  of  sperm,  number  of  sperm,  and  volume  of  semen.  During 
each  collection  performance,  the  activity  and  libido  were  cited. 

The  results  indicated  that  these  breeds  of  rams  cnulU  be  sa- 
tisfactorily divided  into  tivo  groups  according  to  their  reproduc- 
tive capacities  in  the  summer  months,  the  first  group  being  the 
Suffolk  and  Hampshire  breeds  with  the  highest  abilities  and  the 
Shropshires  and  Southdowns  as  the  lovrer-scored  group. 


Tho  Hampshire  and  Suffolk  rams  surpassed  the  Southdo^'Ois  and 
Shropshires  in  motility,  number  of  sperm,  and  libido  throiijhout 
the  experiment.    The  volume  of  semen  per  oolleotion  was  not  signi- 
ficant between  breeds. 

Therefore,  the  conclusions  of  the  exi^eriment  were  tlmt  the  rama 
do  not  seem  to  be  responsible  for  the  late  lambing  seasons,  and  the 
Hampshire  and  Suffolk  rams  would  probably  benefit  the  commercial 
producer  to  a  greater  extent  for  breeding  in  the  sumiaer  months.